The following nomenclature is used: ( FL), fluorescence ( BF), brightfield ( DF), darkfield ( PC), phase contrast ( DIC), differential interference contrast (Nomarski) ( HMC), Hoffman modulation contrast and ( POL), polarized light. Each specimen name includes, in parentheses, an abbreviation designating the contrast mechanism employed in obtaining the image. The tutorial initializes with a randomly selected specimen (imaged in the microscope) appearing in the left-hand window entitled Specimen Image. This interactive tutorial explores the removal of impulse noise from a digital image using the median filter, and how the application of this and related filtering techniques affect the final appearance of the filtered image.
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